Amplified Detection of DNA through an Autocatalytic and Catabolic DNAzyme‐Mediated Process

作者：Fuan Wang, Johann Elbaz, Carsten Teller, Itamar Willner · 发表于：Angewandte Chemie International Edition · 年份：2010 · DOI：10.1002/anie.201005246 · 被引用次数：196 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA and protein synthesis mechanisms、DNA and Nucleic Acid Chemistry

Sense and sensibility: Amplified DNA detection is accomplished by the autocatalytic cleavage of a programmed nucleic acid structure based on the Mg2+-dependent DNAzyme E6 (see picture). The analyte sequence is autocatalytically generated by the DNAzyme from a reporter nucleic acid, which is labeled with a fluorophore and quencher at its two ends.