Isolation, characterization, and nitrification potential of a methylotroph and two heterotrophic bacteria from a consortium showing methane-dependent nitrification

作者：Stanley R. Megraw, Roger Knowles · 发表于：FEMS Microbiology Letters · 年份：1989 · DOI：10.1111/j.1574-6968.1989.tb03392.x · 被引用次数：18 · 研究领域：Microbial metabolism and enzyme function、Wastewater Treatment and Nitrogen Removal、Metalloenzymes and iron-sulfur proteins

A methanotrophic nitrifying consortium was previously obtained from a humisol which showed CH4-dependent nitrification. Although the methanotroph could not be obtained in pure culture, three other members of the consortium have been isolated: An obligately methylotrophic Methylobacillus (Is-1) which grows only on CH3OH and does not nitrify; a Pseudomonas (Is-2) which grows on Is-1 culture filtrate and produces NO2−, NO3− and N2O from NH2OH, and NO3− from NO2−; and a second Pseudomonas (Is-3) which produces NO3− from NH4+ or NO2−, and N2O from NH2OH. A model is proposed for the trophic relations and nitrogen transformations in the consortium which may apply to some natural systems.