1H homonuclear editing of rat brain using semiselective pulses.

作者：Hoby P. Hetherington, Malcolm J. Avison, Robert G. Shulman · 发表于：Proceedings of the National Academy of Sciences · 年份：1985 · DOI：10.1073/pnas.82.10.3115 · 被引用次数：107 · 研究领域：Advanced NMR Techniques and Applications、Advanced MRI Techniques and Applications、NMR spectroscopy and applications

We have used a semiselective Hahn spin-echo sequence of the form (1331)-tau-(2662)-tau-AQ, delivered by a surface coil to obtain high-resolution 1H NMR spectra from the brains of intact dead rats. This sequence gave suppression of the tissue water resonance by a factor of 80,000 when tau = 68 ms. Delivery of a frequency-selective Dante pulse train to the alpha-CH resonance of lactate at 4.11 ppm, simultaneously with the 2662 refocusing pulse, altered the j-modulation in the spin-coupled beta-CH3 protons. Subtraction of this spectrum from one in which the Dante was ineffective gave an edited spectrum containing only the beta-CH3 resonance of lactate at 1.31 ppm. When the position of the Dante was shifted to 3.78 ppm to selectively invert the alpha-CH protons of alanine, an edited spectrum of alanine was obtained.