Stepwise intraclonal maturation of antibody affinity through somatic hypermutation.

作者：Christine Kocks, Klaus Rajewsky · 发表于：Proceedings of the National Academy of Sciences · 年份：1988 · DOI：10.1073/pnas.85.21.8206 · 被引用次数：162 · 研究领域：Monoclonal and Polyclonal Antibodies Research、CAR-T cell therapy research、Transgenic Plants and Applications

Using recombinant DNA techniques, we reconstructed a genealogical tree (Sablitzky, F., Wildner, G. & Rajewsky, K. (1985) EMBO J. 4, 345-350) that connects three clonally related B cells producing somatically mutated antibodies to a progenitor cell expressing a germ line-encoded antibody. The somatic mutants had been isolated from an in vivo immune response. The germ line-encoded progenitor antibody bound the antigen with high affinity. Intraclonal affinity maturation occurred stepwise over a 15-fold range.