Localization of Replicating DNA of Simian Virus 40 in Monkey Kidney Cells

作者：Donald J. LeBlanc, Maxine Singer · 发表于：Proceedings of the National Academy of Sciences · 年份：1974 · DOI：10.1073/pnas.71.6.2236 · 被引用次数：18 · 研究领域：Polyomavirus and related diseases、DNA Repair Mechanisms、Prenatal Screening and Diagnostics

Approximately 80-85% of the DNA synthesized by cells infected with simian virus 40 during a short pulse with [(3)H]thymidine is associated with host nuclear membrane components, as defined by the M-band technique. A substantial portion of this membrane-associated DNA has been shown to be viral specific by sedimentation studies and by DNA.DNA hybridization. Examination of DNA associated and unassociated with nuclear M-band by neutral sucrose gradient analysis suggested that while replicating DNA of simian virus 40 is attached to the nuclear membrane, mature component I DNA of simian virus 40 is released from the membrane upon completion of replication.