The Polymerase Chain Reaction

作者：Jane F. Desforges, Barry I. Eisenstein · 发表于：New England Journal of Medicine · 年份：1990 · DOI：10.1056/nejm199001183220307 · 被引用次数：1170 · 研究领域：T-cell and Retrovirus Studies、CRISPR and Genetic Engineering、Animal Genetics and Reproduction

THE development of molecular genetics, both as a self-contained field and as a body of techniques broadly useful in biologic investigation, has had a profound influence on medical research. The beneficiaries include every discipline in basic science and, at least indirectly, most clinical and applied medical disciplines. Certain technical milestones can be identified over the past several decades that have been particularly important in the progress of the field. One is the discovery of restriction endonucleases, which together with the development of DNA ligation and transformation procedures, led to the ability to clone and thus propagate genes of any organism . . .