Chronic Ethanol Consumption Alters Mammalian Gastrointestinal Content Metabolites

作者：Guoxiang Xie, Wei Hong Zhong, Xiaojiao Zheng, Qiong Li, Yunping Qiu, Houkai Li, Huiyuan Chen, Zhanxiang Zhou, Wei Ping Jia · 发表于：Journal of Proteome Research · 年份：2013 · DOI：10.1021/pr400362z · 被引用次数：140 · 研究领域：Alcohol Consumption and Health Effects、Metabolomics and Mass Spectrometry Studies、Gut microbiota and health

Chronic ethanol consumption is associated with not only the alteration of metabolic profiles in biofluids but also the composition of the gut microbiome. Our understanding of the importance of the intestinal microbiota as well as the disturbances elicited by ethanol intervention is limited by the fact that previous analyses have primarily focused on biofluids and liver tissue metabolome; the metabolic profiles of the gastrointestinal (GI) contents are rarely investigated. In this study, we applied a metabonomics approach using a high performance liquid chromatography-time-of-flight mass spectrometry (HPLC-TOF MS) and gas chromatography-mass spectrometry (GC-MS) to characterize the metabolic alterations of the contents within the GI tract (stomach, duodenum, jejunum, ileum, cecum, colon, and rectum) in male Sprague-Dawley rats following 8 weeks of ethanol exposure. We obtained a snapshot of the distinct changes of the intestinal content metabolite composition in rats with ethanol exposure, which indicated a profound impact of ethanol consumption on the intestinal metabolome. Many metabolic pathways that are critical for host physiology were affected, including markedly altered bile acids, increased fatty acids and steroids, decreased carnitines and metabolites involved in lipid metabolism, a significant decrease of all amino acids and branched chain amino acids, and significantly decreased short chain fatty acids except for acetic acid, which rapidly elevated as a product of e...