Biomimetic pH Sensitive Polymersomes for Efficient DNA Encapsulation and Delivery

作者：Hannah Lomas, Irene Cantón, Sheila MacNeil, Jianzhong Du, Steven P. Armes, Anthony J. Ryan, Andrew L. Lewis, Giuseppe Battaglia · 发表于：Advanced Materials · 年份：2007 · DOI：10.1002/adma.200700941 · 被引用次数：453 · 研究领域：RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques、Polymer Surface Interaction Studies

A novel gene delivery vector based on the ability of a synthetic amphiphilic block copolymer to mimic biological phospholipids by forming membrane-enclosed structures, specifically nanometer-sized vesicles, is presented. A pH-sensitive diblock copolymer forms vesicles (polymersomes) at neutral pH, and dissolves completely as unimers at endocytic pH (see figure). GFP-encoding plasmid DNA are successfully encapsulated inside the polymer vesicles, and delivered intracellularly.