Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

作者：Robert D Fleischmann, Mark D. Adams, Owen R. White, Rebecca A. Clayton, Ewen F. Kirkness, Anthony R. Kerlavage, Carol J. Bult, Jean-François Tomb, Brian Andrew Dougherty, Joseph M. Merrick, Keith H. McKenney, Granger Sutton, Will FitzHugh, Chris Fields, Jeannine D. Gocayne, John L. Scott, Robert B. Shirley, Li-lng Liu, Anna Glodek, Jenny M. Kelley, Janice F. Weidman, Cheryl A. Phillips, Tracy Spriggs, Eva Hedblom, Matthew Cotton, Teresa R. Utterback, Michael C. Hanna, David T. Nguyen, Deborah M. Saudek, Rhonda C. Brandon, Leah D. Fine, Janice L. Fritchman, Joyce L. Fuhrmann, N. S. M. Geoghagen, Cheryl L. Gnehm, Lisa McDonald, Keith V. Small, Claire M. Fraser, Hamilton O. Smith, J. Craig Venter · 发表于：Science · 年份：1995 · DOI：10.1126/science.7542800 · 被引用次数：5664 · 研究领域：Genomics and Phylogenetic Studies、RNA and protein synthesis mechanisms、Biochemical and Structural Characterization

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.