Protein Recognition via Surface Molecularly Imprinted Polymer Nanowires

作者：Yong Li, Huanghao Yang, Qihua You, Zhixia Zhuang, Xiaoru Wang · 发表于：Analytical Chemistry · 年份：2005 · DOI：10.1021/ac050802i · 被引用次数：253 · 研究领域：Analytical chemistry methods development、Mass Spectrometry Techniques and Applications、Microfluidic and Capillary Electrophoresis Applications

In this paper, we present a technique for the preparation of polymer nanowires with the protein molecule imprinted and binding sites at surface. These surface imprinting nanowires exhibit highly selective recognition for a variety of template proteins, including albumin, hemoglobin, and cytochrome c. This recognition may be through a multistep adsorption, with the specificity conferred by hydrogen bonding and shape selectivity. Due to the protein imprinted sites are located at, or close to, the surface; these imprinted nanowires have a good site accessibility toward the target protein molecules. Furthermore, the large surface area of the nanowires results in large protein molecule binding capacity of the imprinted nanowires.