Investigation of analytical variation in metabonomic analysis using liquid chromatography/mass spectrometry

作者：Tim Sangster, Julie Wingate, Lyle Burton, Friederike Teichert, Ian D. Wilson · 发表于：Rapid Communications in Mass Spectrometry · 年份：2007 · DOI：10.1002/rcm.3164 · 被引用次数：57 · 研究领域：Metabolomics and Mass Spectrometry Studies、Analytical Chemistry and Chromatography、Mass Spectrometry Techniques and Applications

Sources of analytical variation in high-performance liquid chromatography/mass spectrometry (HPLC/MS), such as changes in retention, mass accuracy or signal intensity, have been investigated to assess their importance as a variable in the metabonomic analysis of human urine. In this study chromatographic retention and mass accuracy were found to be quite reproducible with the most significant source of analytical variation in the data sets obtained being the result of changes in detector response. Depending on the signal intensity threshold used to define the presence of a peak a sample component could be present in some replicate injections and absent in others within the same run. The implementation of a more sophisticated data software analysis package was found to greatly reduce the impact of detector response variability resulting in improved data analysis.