A sequence-specific DNA-binding protein that activates fushi tarazu segmentation gene expression.

作者：Hitoshi Ueda, Shunro Sonoda, J. Lesley Brown, Matthew P. Scott, Carl C. M. Wu · 发表于：Genes & Development · 年份：1990 · DOI：10.1101/gad.4.4.624 · 被引用次数：189 · 研究领域：Genomics and Chromatin Dynamics、Developmental Biology and Gene Regulation、Animal Genetics and Reproduction

The Drosophila segmentation gene fushi tarazu (ftz) is expressed at the cellular blastoderm stage in a pattern of seven transverse stripes; the stripes lie out of register with the segmental primordia, spanning alternate segmental boundaries. The zebra element, a 740-bp DNA sequence upstream of the ftz translational start, directs striped expression of lacZ when introduced into the fly genome. We have purified to homogeneity a sequence-specific DNA-binding factor, FTZ-F1, that binds to two sites located within the zebra element and to two sites within the ftz protein-coding sequence. FTZ-F1 DNA-binding activity is first detected in extracts of 1.5- to 4-hr embryos, coincident with the time of ftz expression in stripes; the activity then diminishes before reappearing during late embryo, larval, and adult stages. When one of the FTZ-F1-binding sequences in the zebra element is mutated by 2- or 4-base substitutions, the binding to FTZ-F1 is disrupted in vitro, and the intensity of lacZ expression is reduced in transformed embryos, especially in stripes 1, 2, 3, and 6. The results suggest that FTZ-F1 is a transcriptional activator necessary for the proper expression of the ftz gene.