Differentiation of germ cells in seminiferous tubules transplanted to testes of germ cell‐deficient mice of W/Wv and SI/SId genotypes

作者：Hideya Kuroda, Hiroki Nakayama, M Namiki, Keishi Matsumoto, Yoshitake Nishimune, Yukihiko Kitamura · 发表于：Journal of Cellular Physiology · 年份：1989 · DOI：10.1002/jcp.1041390215 · 被引用次数：28 · 研究领域：Sperm and Testicular Function、Reproductive Biology and Fertility、Renal and related cancers

(WB X C57BL/6)F1-W/Wv (hereafter, WBB6F1-W/Wv) mice and (WC X C57BL/6)F1-Sl/Sld (hereafter, WCB6F1-Sl/Sld) mice are sterile due to the deficient spermatogenesis in the testes. The cause of deficient spermatogenesis in WBB6F1-W/Wv mice is considered to be a defect in germ cells themselves, whereas that in WCB6F1-Sl/Sld mice is considered to be a defect in tissue environment necessary for differentiation of germ cells. Seminiferous tubules isolated from cryptorchid testes of C57BL/6- +/+ mice were transplanted into the testes of WBB6F1-W/Wv and WCB6F1-Sl/Sld mice to clarify that the extratubular environment of these mice was intact or not. Type A spermatogonia in the transplanted tubules normally differentiated into spermatids, suggesting that the extratubular environment is intact in both WBB6F1-W/Wv and WCB6F1-Sl/Sld mice.