Monocyte-Macrophage Differentiation Induced by Human Upper Airway Epithelial Cells

作者：Takayuki Ohtoshi, Carlo Vancheri, Gerard Cox, Jack Gauldie, Jerry Dolovich, Judah A. Denburg, Manel Jordana · 发表于：American Journal of Respiratory Cell and Molecular Biology · 年份：1991 · DOI：10.1165/ajrcmb/4.3.255 · 被引用次数：99 · 研究领域：Immune Response and Inflammation、Immunotherapy and Immune Responses、Immune Cell Function and Interaction

Abstract We examined the ability of conditioned medium (CM) generated by human upper airway epithelial (Ep) cells from normal (NN) and inflamed, allergic rhinitis (AR) and nasal polyp (NP) tissues to induce monocytic differentiation of hemopoietic progenitors of the HL-60 myeloid leukemia cell line in vitro. In HL-60 cells cultured in RPMI with 10% FBS, there was differentiation to 0.4 ± 0.4% monocytic cells. NN-, AR-, and NP-EpCM induced differentiation to 23 ± 6%, 42 ± 11%, and 71 ± 10% monocytic cells, respectively. EpCM also induced isolated peripheral blood nonadherent mononuclear cells to express monocyte/macrophage-specific antigens as detected by immunohistochemistry using FMC-32 monoclonal antibodies (anti-CD14). We also examined the cytokine content of these EpCMs and found that they contained granulocyte/macrophage colony-stimulating factor (GM-CSF): 126 ± 35, 198 ± 22, and 489 ± 118 pg/ml for NN-, AR-, and NP-EpCM, respectively. These CMs also contained granulocyte-CSF (G-CSF) and interleukin-6 (IL-6), but there were no significant differences between normal and inflamed tissue-derived cell supernatants. No macrophage-CSF (M-CSF) was detected in these EpCMs. Recombinant human GM-CSF, G-CSF, and IL-6, alone and in combinations, at doses similar to or greater than those found in the EpCMs, did not induce comparable monocytic differentiation of HL-60 cells. Preincubation of the EpCM with neutralizing anti-GM-CSF, anti-G-CSF, or anti-IL-6 antibodies did not significan...