Binding of Drosophila Polo kinase to its regulator Matrimony is noncanonical and involves two separate functional domains

作者：Amanda M. Bonner, Stacie E. Hughes, Jennifer Chisholm, S. Kendall Smith, Brian D. Slaughter, Jay R. Unruh, Kimberly A. Collins, Jennifer M Friederichs, Laurence Florens, Selene K. Swanson, Marissa C. Pelot, Danny E. Miller, Michael P. Washburn, Sue L. Jaspersen, R. Scott Hawley · 发表于：Proceedings of the National Academy of Sciences · 年份：2013 · DOI：10.1073/pnas.1301690110 · 被引用次数：31 · 研究领域：Microtubule and mitosis dynamics、14-3-3 protein interactions、Chromosomal and Genetic Variations

Drosophila melanogaster Polo kinase physically interacts with, and is repressed by, the Matrimony (Mtrm) protein during oogenesis. Females heterozygous for a deletion of the mtrm gene display defects in chromosome segregation at meiosis I. However, a complete absence of Mtrm results in both meiotic catastrophe and female sterility. We show that three phosphorylated residues in an N-terminal region in Mtrm are required for Mtrm::Polo binding. However, this binding is noncanonical; it does not require either a complete S-pS/pT-P motif in Mtrm or key residues in the Polo-box domain of Polo that allow Polo to bind phosphorylated substrates. By using fluorescence cross-correlation spectroscopy to characterize the Mtrm::Polo interaction in vivo, we show that a sterile α-motif (SAM) domain located at the C terminus of Mtrm increases the stability of Mtrm::Polo binding. Although Mtrm's C-terminal SAM domain is not required to rescue the chromosome segregation defects observed in mtrm/+ females, it is essential to prevent both meiotic catastrophe and the female sterility observed in mtrm/mtrm females. We propose that Polo's interaction with the cluster of phosphorylated residues alone is sufficient to rescue the meiosis I defect. However, the strengthening of Mtrm::Polo binding mediated by the SAM domain is necessary to prevent meiotic catastrophe and ensure female fertility. Characterization of the Mtrm::Polo interaction, as well as that of other Polo regulators, may assist in the de...