Confocal differential interference contrast (DIC) microscopy: including a theoretical analysis of conventional and confocal DIC imaging

作者：Carol J. Cogswell, Colin J. R. Sheppard · 发表于：Journal of Microscopy · 年份：1992 · DOI：10.1111/j.1365-2818.1992.tb04307.x · 被引用次数：121 · 研究领域：Optical Coherence Tomography Applications、Advanced X-ray Imaging Techniques、Digital Holography and Microscopy

SUMMARY A technique for obtaining differential interference contrast (DIC) imaging using a confocal microscope system is examined and its features compared to those of existing confocal differential phase contrast (DPC) techniques as well as to conventional Nomarski DIC. A theoretical treatment of DIC imaging is presented, which takes into account the vignetting effect caused by the finite size of the lens pupils. This facilitates the making of quantitative measurements in DIC and allows the user to identify and select the most appropriate system parameters, such as the bias retardation and lateral shear of the Wollaston prism.