Molecular Cloning of Human and Rat Complementary DNA Encoding Androgen Receptors

作者：Chawnshang S. Chang, John M. Kokontis, Shutsung Liao · 发表于：Science · 年份：1988 · DOI：10.1126/science.3353726 · 被引用次数：913 · 研究领域：Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities、Hormonal and reproductive studies、Estrogen and related hormone effects

Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA-binding domain that is highly conserved in all steroid receptors. The human cDNA was transcribed and the RNA product was translated in cell-free systems to yield a 76-kilodalton protein. The protein was immunoprecipitable by human autoimmune antibodies to the androgen receptor. The protein bound androgens specifically and with high affinity.