Visualization of chromatin substructure: upsilon bodies.

作者：Ada L. Olins, Robert D. Carlson, Donald E. Olins · 发表于：The Journal of Cell Biology · 年份：1975 · DOI：10.1083/jcb.64.3.528 · 被引用次数：101 · 研究领域：Nuclear Structure and Function、DNA and Nucleic Acid Chemistry、DNA Repair Mechanisms

Spread chromatin fibers, from isolated eucaryotic nuclei, reveal linear arrays of spherical particles (upsilon bodies), about 70 A in diameter, connected by thin filaments about 15 A wide. These particles have been observed in freshly isolated nuclei from rat thymus, rat liver, and chicken erythrocytes. In addition, upsilon bodies can be visualized in preparations of isolated sheared chromatin, and in chromatin reconstructed from dissociating solvent conditions (i.e., high urea-NaCl concentration). As a criterion for perturbation of native chromatin structure low-angle X-ray diffraction patterns were obtained from nuclear pellets at different stages in the preparation of nuclei fro electron microscopy. These results suggest that the particulate (upsilon body) structures observed by electron microscopy may be closely related to the native configuration of chromatin.