Two Different cis -Active Elements Transfer the Transcriptional Effects of Both EGF and Phorbol Esters

作者：Harry P. Elsholtz, Harry J. Mangalam, Ellen Potter, Vivian R. Albert, Scott C. Supowit, Ronald M. Evans, Michael G. Rosenfeld · 发表于：Science · 年份：1986 · DOI：10.1126/science.3491428 · 被引用次数：160 · 研究领域：Genomics and Chromatin Dynamics、RNA Research and Splicing、RNA and protein synthesis mechanisms

Short cis-active sequences of the rat prolactin or Moloney murine leukemia virus genes transfer transcriptional regulation by both epidermal growth factor and phorbol esters to fusion genes. These sequences act in a position- and orientation-independent manner. Competitive binding analyses with nuclear extracts from stimulated and unstimulated cells suggest that different trans-acting factors associate with the regulatory sequence of each gene. A model is proposed suggesting that both epidermal growth factor and phorbol esters stimulate the transcription of responsive genes via discrete classes of hormone-dependent, enhancer-like elements that bind different trans-acting factors, even in the absence of hormone stimulation.