Ethylene-Binding Sites Generated in Yeast Expressing the Arabidopsis ETR1 Gene

作者：G. Eric Schaller, Anthony B. Bleecker · 发表于：Science · 年份：1995 · DOI：10.1126/science.270.5243.1809 · 被引用次数：624 · 研究领域：Postharvest Quality and Shelf Life Management、Plant Physiology and Cultivation Studies、Nanocomposite Films for Food Packaging

Mutations in the ETR1 gene of Arabidopsis thaliana confer insensitivity to ethylene, which indicates a role for the gene product in ethylene signal transduction. Saturable binding sites for [14C]ethylene were detected in transgenic yeast expressing the ETR1 protein, whereas control yeast lacking ETR1 showed no detectable ethylene binding. Yeast expressing a mutant form of ETR1 (etr1-1) also showed no detectable ethylene binding, which provides an explanation for the ethylene-insensitive phenotype observed in plants carrying this mutation. Expression of truncated forms of ETR1 in yeast provided evidence that the amino-terminal hydrophobic domain of the protein is the site of ethylene binding. It was concluded from these results that ETR1 acts as an ethylene receptor in Arabidopsis.