In Vitro and In Vivo Effect of Interleukin-2 on the 2′,5′-Oligoadenylate Synthetase Activity of Peripheral Mononuclear Blood Cells

作者：Rupert Handgretinger, Gernot Bruchelt, Astrid Kimmig, Puyi Lang, B Daurer, R. Dopfer, J. Treuner, D. Niethammer · 发表于：Journal of Interferon Research · 年份：1990 · DOI：10.1089/jir.1990.10.75 · 被引用次数：5 · 研究领域：Immune Cell Function and Interaction、Immune Response and Inflammation、Immune cells in cancer

The in vitro and in vivo influence of interleukin-2 (IL-2) on 2',5'-oligoadenylate (2-5A) synthetase activity and natural killer (NK) activity of peripheral mononuclear blood cells (PBMCs) was investigated. Incubation of PBMCs in vitro with IL-2 resulted in a considerable secretion of interferon-gamma (IFN-gamma) and in a significant elevation of 2-5A synthetase activity, as well as NK activity. Neutralizing monoclonal anti-IFN-gamma antibodies inhibited the elevation of 2-5A synthetase activity, but not the IL-2-induced augmentation of NK activity. Additionally, 2-5A synthetase and NK activity of PBMCs was measured in a child with neuroblastoma that was treated with recombinant IL-2 by continuous intravenous application. During the treatment, NK activity against the NK-sensitive cell line K 562 and against autologous tumor cells was not augmented. However, a significant increase of 2-5A synthetase activity in PBMCs was observed during IL-2 treatment, whereas there was no detectable serum level of IFN-gamma. We conclude that measuring 2-5A synthetase activity in patients treated with IL-2 may be helpful in monitoring the immunomodulatory effects of IL-2 on immune effector cells.