Movement of Eukaryotic mRNAs Between Polysomes and Cytoplasmic Processing Bodies

作者：Muriel Brengues, Daniela Teixeira, Roy Parker · 发表于：Science · 年份：2005 · DOI：10.1126/science.1115791 · 被引用次数：810 · 研究领域：RNA Research and Splicing、RNA and protein synthesis mechanisms、RNA modifications and cancer

Eukaryotic cells contain nontranslating messenger RNA concentrated in P-bodies, which are sites where the mRNA can be decapped and degraded. We present evidence that mRNA molecules within yeast P-bodies can also return to translation. First, inhibiting delivery of new mRNAs to P-bodies leads to their disassembly independent of mRNA decay. Second, P-bodies decline in a translation initiation-dependent manner during stress recovery. Third, reporter mRNAs concentrate in P-bodies when translation initiation is blocked and resume translation and exit P-bodies when translation is restored. Fourth, stationary phase yeast have large P-bodies containing mRNAs that reenter translation when growth resumes. The reciprocal movement of mRNAs between polysomes and P-bodies is likely to be important in the control of mRNA translation and degradation. Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage.