Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation

作者：Susan C. Thornton, Stephen N. Mueller, Elliot M. Levine · 发表于：Science · 年份：1983 · DOI：10.1126/science.6635659 · 被引用次数：801 · 研究领域：Angiogenesis and VEGF in Cancer、3D Printing in Biomedical Research、Mesenchymal stem cell research

Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the first time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.