Deciphering Thylakoid Sub-compartments using a Mass Spectrometry-based Approach

作者：Martino Tomizioli, Cosmin Lazar, Sabine Brugière, Thomas Bürger, Daniel Salvi, Laurent Gatto, Lucas Moyet, Lisa M. Breckels, Anne-Marie Hesse, Kathryn Susan Lilley, Daphné Seigneurin‐Berny, Giovanni Finazzi, Norbert Rolland, Myriam Ferro · 发表于：Molecular & Cellular Proteomics · 年份：2014 · DOI：10.1074/mcp.m114.040923 · 被引用次数：102 · 研究领域：Photosynthetic Processes and Mechanisms、Metal-Catalyzed Oxygenation Mechanisms、Algal biology and biofuel production

Photosynthesis has shaped atmospheric and ocean chemistries and probably changed the climate as well, as oxygen is released from water as part of the photosynthetic process. In photosynthetic eukaryotes, this process occurs in the chloroplast, an organelle containing the most abundant biological membrane, the thylakoids. The thylakoids of plants and some green algae are structurally inhomogeneous, consisting of two main domains: the grana, which are piles of membranes gathered by stacking forces, and the stroma-lamellae, which are unstacked thylakoids connecting the grana. The major photosynthetic complexes are unevenly distributed within these compartments because of steric and electrostatic constraints. Although proteomic analysis of thylakoids has been instrumental to define its protein components, no extensive proteomic study of subthylakoid localization of proteins in the BBY (grana) and the stroma-lamellae fractions has been achieved so far. To fill this gap, we performed a complete survey of the protein composition of these thylakoid subcompartments using thylakoid membrane fractionations. We employed semiquantitative proteomics coupled with a data analysis pipeline and manual annotation to differentiate genuine BBY and stroma-lamellae proteins from possible contaminants. About 300 thylakoid (or potentially thylakoid) proteins were shown to be enriched in either the BBY or the stroma-lamellae fractions. Overall, present findings corroborate previous observations obtain...