Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana

作者：Clare Lister, Caroline Dean · 发表于：The Plant Journal · 年份：1993 · DOI：10.1046/j.1365-313x.1993.04040745.x · 被引用次数：573 · 研究领域：Plant Pathogens and Resistance、Plant Disease Resistance and Genetics、Plant Molecular Biology Research

Recombinant inbred (RI) lines of Arabidopsis thaliana (Arabidopsis) have been generated from a cross between the ecotypes Landsberg erecta and Columbia. Progeny of 300 individual F2 seedlings were taken by single seed descent to the F8 generation. Sixty‐seven loci, scored using 64 RFLP probes and one phenotypic marker, chosen at approximately 20 cM intervals from the two previously published RFLP maps, were mapped using 100 of these RI lines. More than 500 other new loci are currently being mapped using these RI lines by several other groups. These 100 RI lines thus provide the material to map new probes or phenotypic traits polymorphic between Landsberg erecta and Columbia, relative to an increasing number of molecular markers. Higher resolution mapping of distinct chromosomal regions can be achieved by analysing the segregation of particular markers on the additional 200 RI lines.