Reciprocal expression of human ETS1 and ETS2 genes during T-cell activation: regulatory role for the protooncogene ETS1.

作者：N K Bhat, Craig B. Thompson, Tullia Lindsten, Carl H. June, Shigeyoshi Fujiwara, Shigeki Koizumi, Robert J. Fisher, Takis S. Papas · 发表于：Proceedings of the National Academy of Sciences · 年份：1990 · DOI：10.1073/pnas.87.10.3723 · 被引用次数：147 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Immunotherapy and Immune Responses、Complement system in diseases

The expression of the protooncogenes ETS1 and ETS2 has been studied in purified human T cells activated either by cross-linking of the T-cell receptor-CD3 complex on their cell surface or by direct stimulation with phorbol esters and ionomycin. Our results show that resting T cells express high levels of ETS1 mRNA and protein, while expression of ETS2 is undetectable. Upon T-cell activation, ETS2 mRNA and proteins are induced, while ETS1 gene expression decreases to very low levels. Late after stimulation, ETS1 mRNA is reinduced and maintained at a high level, while ETS2 gene expression decreases to undetectable levels. Therefore, it appears that in human T cells, ETS2 gene products are associated with cellular activation and proliferation, while ETS1 gene products are preferentially expressed in a quiescent state.