Relationship between diminished ovarian reserve and mitochondrial biogenesis in cumulus cells

作者：Lisa Boucret, Juan Manuel Chao de la Barca, Catherine Morinière, Valérie Desquiret‐Dumas, Véronique Ferré-L’Hôtellier, Philippe Descamps, Charles Marcaillou, Pascal Reynier, Vincent Procaccio, Pascale May‐Panloup · 发表于：Human Reproduction · 年份：2015 · DOI：10.1093/humrep/dev114 · 被引用次数：193 · 研究领域：Reproductive Biology and Fertility、Ovarian function and disorders、Sperm and Testicular Function

STUDY QUESTION: What part do mitochondria play in cases of diminished ovarian reserve (DOR)? SUMMARY ANSWER: Mitochondrial biogenesis in cumulus cells may be linked with impaired oocyte competence in patients with DOR. WHAT IS KNOWN ALREADY: DOR, one of the causes of infertility even in young women, is characterized by the depletion of the ovarian pool associated with a decline in oocyte competence. Mitochondria, which play a role in oocyte quality, could be involved in the pathogenesis of DOR. The study of cumulus cells offers an interesting non-invasive approach for evaluating oocyte quality and the metabolic processes on which it depends. If mitochondrial dysfunction is involved in DOR, it is likely to have an impact on the functioning of cumulus cells. STUDY DESIGN, SIZE, DURATION: This is an observational study of 74 immature oocyte-cumulus complexes retrieved from 47 women undergoing in vitro fertilization with intracytoplasmic sperm injection at the University Hospital of Angers, France, from March 2013 to March 2014. The women were divided into two groups: one group included 26 women with DOR, and the other, which included 21 women with a normal ovarian reserve (NOR), served as a control group. PARTICIPANTS/MATERIALS, SETTINGS, METHODS: The oocyte mitochondrial content and the average mitochondrial content of the cumulus cells were assessed by mitochondrial (mt)DNA quantification using a quantitative real-time PCR technique. Microfluidic-based quantitative RT-PCR assa...