Effects of normobaric oxygen and melatonin on reperfusion injury: role of cerebral microcirculation

作者：Mustafa Çağlar Beker, Ahmet B. Çağlayan, Taha Keleştemur, Berrak Çağlayan, Esra Yalçın, Burak Yuluğ, Ülkan Kılıç, Dirk Matthias Hermann, Ertuğrul Kılıç · 发表于：Oncotarget · 年份：2015 · DOI：10.18632/oncotarget.5773 · 被引用次数：61 · 研究领域：Circadian rhythm and melatonin、Thermoregulation and physiological responses、Optical Imaging and Spectroscopy Techniques

// Mustafa C. Beker 1 , Ahmet B. Caglayan 1 , Taha Kelestemur 1 , Berrak Caglayan 1 , Esra Yalcin 1 , Burak Yulug 2 , Ulkan Kilic 1 , Dirk M. Hermann 3 and Ertugrul Kilic 1 1 Department of Physiology and Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Istanbul, Turkey 2 Department of Neurology, Istanbul Medipol University, Istanbul, Turkey 3 Department of Neurology, University Hospital Essen, Essen, Germany Correspondence to: Ertugrul Kilic, email: // Keywords : hyperoxia, reperfusion injury, cerebral microcirculation, BBB permeability, NOS, Pathology Section Received : August 14, 2015 Accepted : September 13, 2015 Published : September 21, 2015 Abstract In order to protect the brain before an irreversible injury occurs, penumbral oxygenation is the primary goal of current acute ischemic stroke treatment. However, hyperoxia treatment remains controversial due to the risk of free radical generation and vasoconstriction. Melatonin is a highly potent free radical scavenger that protects against ischemic stroke. Considering its anti-oxidant activity, we hypothesized that melatonin may augment the survival-promoting action of normobaric oxygen (NBO) and prevent brain infarction. Herein, we exposed mice to 30 or 90 min of intraluminal middle cerebral artery occlusion (MCAo) and evaluated the effects of NBO (70% or 100% over 90 min), administered either alone or in combination with melatonin (4 mg/kg, i.p.), on disseminate neuronal injury, neurolog...