Transformation of Salmonella typhimurium by Plasmid Deoxyribonucleic Acid

作者：Esther M. Lederberg, Stanley N. Cohen · 发表于：Journal of Bacteriology · 年份：1974 · DOI：10.1128/jb.119.3.1072-1074.1974 · 被引用次数：767 · 研究领域：Bacteriophages and microbial interactions、CRISPR and Genetic Engineering、Bacterial Genetics and Biotechnology

A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristics of the transforming plasmid.