Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum

作者：Miles D. Savage, Zhigang Wu, Steven L. Daniel, Leon L. Lundie, Harold L. Drake · 发表于：Applied and Environmental Microbiology · 年份：1987 · DOI：10.1128/aem.53.8.1902-1906.1987 · 被引用次数：78 · 研究领域：Hemoglobin structure and function、Microbial bioremediation and biosurfactants、Photosynthetic Processes and Mechanisms

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO----CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO. Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.