Structures of the Sugar Chains of Recombinant Human Granulocyte-Colony-Stimulating Factor Produced by Chinese Hamster Ovary Cells

作者：Masayoshi Oh‐eda, Sumihiro Hase, Masayoshi Ono, Tokuji Ikenaka · 发表于：The Journal of Biochemistry · 年份：1988 · DOI：10.1093/oxfordjournals.jbchem.a122305 · 被引用次数：75 · 研究领域：Viral Infectious Diseases and Gene Expression in Insects、RNA Interference and Gene Delivery、Immune Cell Function and Interaction

Recombinant human granulocyte-colony-stimulating factor (G-CSF) was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA. The recombinant human G-CSF was treated with alkaline borohydride and the oligosaccharide-alditols liberated were fractioned by gel filtration on a Bio-Gel P-4 column, followed by high-performance liquid chromatography by use of a strong anion exchanger. Two oligosaccharide-alditols were obtained and their structures were identified by component analysis and 500-MHz 1H-NMR spectroscopy. The structures of the sugar chains were NeuAc alpha 2-3Gal beta 1-3GalNAcol and NeuAc alpha 2-3Gal beta 1-3(NeuAc alpha 2-6)GalNAcol.