Measurement of Antibodies to Insulin in Serum

作者：Kathryn Dixon · 发表于：Clinical Chemistry · 年份：1974 · DOI：10.1093/clinchem/20.10.1275 · 被引用次数：57 · 研究领域：Diabetes Management and Research、Pancreatic function and diabetes、Metabolism, Diabetes, and Cancer

Abstract I describe a method for measuring insulin antibodies in insulin-treated subjects. The antibodies are characterized by maximum binding capacity and affinity constant. Endogenous and therapeutic insulin interferes in insulin antibody assay, and it is removed by dextran—charcoal treatment at pH 3.5. Endogenous total and free insulin may be measured by a simple extension of the method.