Addi-Chek filtration, BACTEC, and 10-ml culture methods for recovery of microorganisms from dialysis effluent during episodes of peritonitis

作者：B M Males, J. Joseph Walshe, L Garringer, D Koscinski, Daniel Amsterdam · 发表于：Journal of Clinical Microbiology · 年份：1986 · DOI：10.1128/jcm.23.2.350-353.1986 · 被引用次数：32 · 研究领域：Dialysis and Renal Disease Management、Bacterial Identification and Susceptibility Testing、Urinary Tract Infections Management

The Addi-Chek (filtration; Millipore Corp., Bedford, Mass.) and BACTEC (radiometric detection of growth in culture media; Johnston Laboratories, Inc., Towson, Md.) systems were compared with the 10-ml culture (centrifugation) method for the recovery of microorganisms from peritoneal dialysate collected from patients with clinical evidence of peritonitis and containing greater than or equal to 200 leukocytes per mm3. Both alternate methods were comparable, and results were not significantly different from those of the conventional 10-ml culture method. All systems were adversely affected in their capacity to recover organisms when dialysates had been collected during periods of antimicrobial therapy.