High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA

作者：Daniel A. Simon, Anne-Elizabeth Rouault, Marie‐Christine Chopin · 发表于：Applied and Environmental Microbiology · 年份：1986 · DOI：10.1128/aem.52.2.394-395.1986 · 被引用次数：63 · 研究领域：Probiotics and Fermented Foods、RNA and protein synthesis mechanisms、Genomics and Phylogenetic Studies

Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).