New Fluorogenic Substrates for α-Thrombin, Factor Xa, Kallikreins, and Urokinase1

作者：Takashi Morita, Hisao Kato, Sadaaki Iwanaga, Katsumi Takada, Terutoshi Kimura, Shumpei Sakakibara · 发表于：The Journal of Biochemistry · 年份：1977 · DOI：10.1093/oxfordjournals.jbchem.a131840 · 被引用次数：384 · 研究领域：Blood Coagulation and Thrombosis Mechanisms、Coagulation, Bradykinin, Polyphosphates, and Angioedema、Hemophilia Treatment and Research

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.