Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans

作者：Paula Sundstrom, Everett J. Nichols, G E Kenny · 发表于：Infection and Immunity · 年份：1987 · DOI：10.1128/iai.55.3.616-620.1987 · 被引用次数：60 · 研究领域：Antifungal resistance and susceptibility、Carbohydrate Chemistry and Synthesis、Glycosylation and Glycoproteins Research

To determine the nature of germ tube-specific antigens of Candida albicans, procedures for intrinsically labeling cell wall antigens metabolically were developed. Blastospores or germ tubes labeled either in their proteins with L-[35S]methionine or in mannose-containing carbohydrates with D[2-3H]mannose contained surface components similar to those found previously with 125I-labeled organisms. Germ tube-specific determinants, were found on a 200-kilodalton protein in digests from germ tubes, whereas a component of similar molecular size in blastospore extracts reacted weakly or not at all with germ tube-specific antibody. In addition, a glycan fraction prepared from germ tubes reacted with the unadsorbed anti-C. albicans polyvalent antibody but not with the germ tube-specific antibody, suggesting that the germ tube-specific determinants are not carbohydrates.