Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry

作者：Andreas Dominik Braun, Daniel P. Little, Hubert Köster · 发表于：Clinical Chemistry · 年份：1997 · DOI：10.1093/clinchem/43.7.1151 · 被引用次数：177 · 研究领域：Cystic Fibrosis Research Advances、Advanced biosensing and bioanalysis techniques、Bacterial Genetics and Biotechnology

A new method for the reliable identification of localized variations in DNA by detection of associated diagnostic products with matrix-assisted laser desorption ionization time-of-flight mass spectrometry is described. The diagnostic products are generated by the primer oligo base extension (PROBE) reaction, which requires a single detection primer complementary to a region down-stream of a target strand's variable site. On addition of a polymerase, three dNTPs, and the fourth nucleotide in dideoxy form, the primer is extended through the mutation region until the first ddNTP is incorporated; the mass of the extension products determines the composition of the variable site. Tests for five cystic fibrosis mutations, including two exon 11 sites measured in a biplex reaction, and for differentiating between three common alleles of the poly(T) tract at the intron 8 splice acceptor site of the CFTR gene are presented. All experimental steps required for PROBE are amenable to the high degree of automation desirable for a high-through-put diagnostic setting. Furthermore, it requires no fluorescent, chemiluminescent, or radioactive labeling; the mass signals measured offer a far more analytically definitive signal, leading in all cases to high-quality unambiguous and easily interpreted results.