Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori

作者：James A. Versalovic, Dee Shortridge, K. Kibler, M. V. Griffy, Jill M. Beyer, Robert K. Flamm, Shinji Tanaka, David Yates Graham, M. F. Go · 发表于：Antimicrobial Agents and Chemotherapy · 年份：1996 · DOI：10.1128/aac.40.2.477 · 被引用次数：505 · 研究领域：Helicobacter pylori-related gastroenterology studies、Veterinary medicine and infectious diseases、Gastric Cancer Management and Outcomes

Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of 23S rRNA. A-->G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.