Automated Determination of Serum Iron

作者：Bennie Zak, Emanuel Z. Epstein · 发表于：Clinical Chemistry · 年份：1965 · DOI：10.1093/clinchem/11.6.641 · 被引用次数：46 · 研究领域：Iron Metabolism and Disorders

Abstract An automated procedure for measurement of serum iron concentration is presented. Iron released from the serum transferrin by acid treatment in the presence of ascorbic acid was dialyzed into a buffer capable of maintaining the diffusate at a pH suitable for instantaneous and complete color formation with 4,7-diphenyl-1,10 phenanthroline sulfonate. Procedural details, diffusability of iron, precision, and accuracy are described.