The abundant retinal protein of the Chlamydomonas eye is not the photoreceptor for phototaxis and photophobic responses

作者：Markus Fuhrmann, Alke Stahlberg, Elena G. Govorunova, Steve Rank, Peter Hegemann · 发表于：Journal of Cell Science · 年份：2001 · DOI：10.1242/jcs.114.21.3857 · 被引用次数：117 · 研究领域：Photoreceptor and optogenetics research、Photosynthetic Processes and Mechanisms、Light effects on plants

The chlamyopsin gene (cop) encodes the most abundant eyespot protein in the unicellular green alga Chlamydomonas reinhardtii. This opsin-related protein (COP) binds retinal and was thought to be the photoreceptor controlling photomovement responses via a set of photoreceptor currents. Unfortunately, opsin-deficient mutants are not available and targeted disruption of non-selectable nuclear genes is not yet possible in any green alga. Here we show that intron-containing gene fragments directly linked to their intron-less antisense counterpart provide efficient post-transcriptional gene silencing (PTGS) in C. reinhardtii, thus allowing an efficient reduction of a specific gene product in a green alga. In opsin-deprived transformants, flash-induced photoreceptor currents (PC) are left unchanged. Moreover, photophobic responses as studied by motion analysis and phototaxis tested in a light-scattering assay were indistinguishable from the responses of untransformed wild-type cells. We conclude that phototaxis and photophobic responses in C. reinhardtii are triggered by an as yet unidentified rhodopsin species.