Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans

作者：Louis A. Sherman, Piet van de Putte · 发表于：Journal of Bacteriology · 年份：1982 · DOI：10.1128/jb.150.1.410-413.1982 · 被引用次数：45 · 研究领域：CRISPR and Genetic Engineering、Protist diversity and phylogeny、Advanced biosensing and bioanalysis techniques

A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicated in both organisms, had unique sites for two restriction endonucleases, conferred ampicillin resistance on both organisms, and could be used as a cloning vector in A. nidulans.