GATA-3 Directly Remodels the IL-10 Locus Independently of IL-4 in CD4+ T Cells

作者：John Shoemaker, Margarida Saraiva, Anne O’Garra · 发表于：The Journal of Immunology · 年份：2006 · DOI：10.4049/jimmunol.176.6.3470 · 被引用次数：138 · 研究领域：Immune Cell Function and Interaction、Immune Response and Inflammation、Immunotherapy and Immune Responses

IL-10 is a major regulator in inflammatory responses. Although various transcription factors were defined to enhance IL-10, the molecular mechanism for the initiation of Il-10 transcription, remains unknown. mRNA profiling of six distinct primary CD4+ T cell populations showed differential expression of the transcription factor GATA-3 correlated with levels of IL-10 expression. We showed that ectopic expression of GATA-3 in naive primary CD4+ T cells enhanced expression of IL-10 by these cells and uncovered a possible mechanism for this effect. We found that GATA-3 induced changes of the chromatin structure at the Il-10 locus and that these changes occur even in the absence of IL-4. Furthermore we found that in the presence of GATA-3 the histones at the Il-10 locus become acetylated. Despite being recruited in vivo to two locations on the Il-10 locus, GATA-3 did not transactivate the IL-10 promoter. We therefore suggest a key role of GATA-3 in instructing Il-10 gene expression in primary CD4+ T cells, possibly by switching and stabilizing the Il-10 locus into a transcriptionally competent status.