A Description of the Technique for Nuclear Transplantation inXenopus laevis

作者：Tom Elsdale, John Bertrand Gurdon, Michail Fischberg · 发表于：Development · 年份：1960 · DOI：10.1242/dev.8.4.437 · 被引用次数：101 · 研究领域：Renal and related cancers、Reproductive Biology and Fertility

ABSTRACT Technical details are given of a method of transplanting embryonic nuclei in X. laevis. This technique is based on Briggs & King’s method for R. pipiens. Owing to certain differences between the eggs of Rana and Xenopus, their technique has been modified for Xenopus. The main modification for Xenopus concerns the use of ultra-violet irradiation instead of enucleation by a needle. Differences between transplantation techniques for Rana and Xenopus are unlikely to account for the differences in experimental results obtained with these species.