Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA

作者：Roland Weller, David M. Ward · 发表于：Applied and Environmental Microbiology · 年份：1989 · DOI：10.1128/aem.55.7.1818-1822.1989 · 被引用次数：114 · 研究领域：Microbial Community Ecology and Physiology、Environmental DNA in Biodiversity Studies、Genomics and Phylogenetic Studies

Cloning of cDNA obtained from 16S rRNA (16S rcDNA) selectively retrieves species-specific sequence information useful for analyzing the composition and structure of natural microbial communities. With this technique we obtained recombinant 16S rcDNA libraries from Escherichia coli and from a model hot-spring cyanobacterial-mat community. The recombinant plasmids contained exclusively 16S rRNA-derived inserts. This selective approach is independent of biasing culture techniques and eliminates the laborious screening required to locate 16S rRNA gene-bearing recombinants in genomic DNA libraries obtained from natural communities.