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Application of a Microtechnique to Viral Serological Investigations

作者:John L. Sever · 发表于:The Journal of Immunology · 年份:1962 · DOI:10.4049/jimmunol.88.3.320 · 被引用次数:1623 · 研究领域:Blood groups and transfusion、Parvovirus B19 Infection Studies、Respiratory viral infections research

Summary A microtechnique (modified Takatsy) is described which can be applied to complement fixation, hemagglutination, hemagglutination inhibition and metabolic inhibition tests. The system permits an 8-fold saving of reagents and rapid performance of microdilutions. The specific methods and modifications of equipment necessary to obtain reliable results are presented in detail. Comparative data obtained with the micro- and standard systems establish the reliability and validity of the microsystem.