Effect of avermectin (AVM) on the expression of γ -aminobutyric acid A receptor (GABAA R) in Carassius gibelio (Bloch, 1782)

作者：A. Zhou, Kun Hu, Jiming Ruan, Haijing Cao, Yinghui Wang, Yifan Zhao, X. Yang · 发表于：Journal of Applied Ichthyology · 年份：2015 · DOI：10.1111/jai.12794 · 被引用次数：1 · 研究领域：Parasite Biology and Host Interactions、Neurobiology and Insect Physiology Research、Neuroendocrine regulation and behavior

This study describes the effect of avermectin (AVM) on the expression of γ-aminobutyric acid A receptor (GABAAR) in Carassius gibelio. To assess the specific expression of GABAAR in the brain, gonads, liver, kidneys, heart, muscles, and skin of C. gibelio, the expression of GABAAR α1 subunit (GABAARα1) was measured by Western blotting. To study the effects of AVM on the expression of GABAAR, the median lethal concentration (LC50) at 24, 48, and 96 h of AVM was determined and the expression of GABAAR in the brain, liver, and kidneys of the corresponding C. gibelio evaluated by Western blotting and immunohistochemistry. The results show that GABAAR was expressed in the brain, gonads, liver, kidneys, heart, intestines, muscles, and skin, while primarily distributed in the central nervous system and moderately distributed in peripheral tissues. The expression of GABAAR in the brain, liver, and kidney tissues of C. gibelio was increased with the treatment of AVM at 24 h LC50, but attenuated by the treatment of AVM at 48 h LC50 and 96 h LC50. This suggests a threshold effect of AVM.