Characterization of nuclear structures containing superhelical DNA

作者：Peter Richard Cook, I. A. Brazell, Erich Jost · 发表于：Journal of Cell Science · 年份：1976 · DOI：10.1242/jcs.22.2.303 · 被引用次数：370 · 研究领域：DNA and Nucleic Acid Chemistry、Genomics and Chromatin Dynamics、DNA Repair Mechanisms

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.