The preparation and properties of β-glucuronidase. 4. Inhibition by sugar acids and their lactones

作者：G. A. Levvy · 发表于：Biochemical Journal · 年份：1952 · DOI：10.1042/bj0520464 · 被引用次数：315 · 研究领域：Carbohydrate Chemistry and Synthesis、Glycosylation and Glycoproteins Research、Natural Antidiabetic Agents Studies

doubt, prove very useful in certain investigations owing to its high degree of sensitivity.The wide variations of both the acid number and the iodine number indicate variations in the composition ofsebum.Squalene is the most unsaturated constituent of sebum and small changes in its concentration would produce wide fluctuation in the iodine number.This and other variations in the composition ofsebum are now being studied in order to obtain further information about certain skin diseases.SUMMARY 1. Methods available for the collection and estimation of sebum have been studied in order to assess their reliability.2. The cup method of collection has been found satisfactory when carbon tetrachloride is used as solvent.3. Direct weighing of the sebum sample is recommended, but reliable results can be obtained with either the nephelometric or the chromate- oxidation method provided that the method has previously been calibrated by means of samples of sebum.The authors wish to thank Dr R. M. B. MacKenna for his interest in this work and Prof. A. Wormall for much helpful advice and criticism.They also wish to thank the medical students of this College from whom sebum specimens