The Interplay Between miR-148a and DNMT1 Might be Exploited for Pancreatic Cancer Therapy

作者：Qian Zhan, Yuan Fang, Xiaxing Deng, Hao Chen, Jianbin Jin, Xiongxiong Lu, Chenghong Peng, Hongwei Li, Baiyong Shen · 发表于：Cancer Investigation · 年份：2015 · DOI：10.3109/07357907.2015.1025794 · 被引用次数：26 · 研究领域：MicroRNA in disease regulation、Epigenetics and DNA Methylation、RNA modifications and cancer

We discovered the expression level of miR-148a significantly decreased in pancreatic cancer tissues whereas that of DNMT1 increased. In ASPC-1 cancer cells, the overexpression of miR-148a led to a decreased level of DNMT1 and reduced the proliferation and metastasis of ASPC-1 cells. Moreover, the increased expression of miR-148a arrested the UTR methylation of p27, giving rise to an increased level of p27. Interestingly, it was shown that the DNMT1 inhibition enhanced the expression of miR-148a. In vivo studies demonstrated that the tumorigenesis of ASPC-1 was significantly arrested by either the overexpression of miR-148a or the inhibition of DNMT1.