Processing of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear, single-stranded T-DNA

作者：Lisa M. Albright, M. Yanofsky, B. Leroux, D Q, Eugene W. Nester · 发表于：Journal of Bacteriology · 年份：1987 · DOI：10.1128/jb.169.3.1046-1055.1987 · 被引用次数：157 · 研究领域：Plant tissue culture and regeneration、Chromosomal and Genetic Variations、Polyamine Metabolism and Applications

Transfer and integration of a defined region (T-DNA) of the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens is essential for tumor formation. We used a physical assay to study structural changes induced in Agrobacterium T-DNA by cocultivation with plant cells. We show that nicks are introduced at unique, identical locations in each of the 24-base-pair imperfect direct repeats which flank the T-DNA and present evidence that a linear, single-stranded molecule is generated. We propose that these changes result from processing of the T-DNA for transfer and that they occur by a mechanism similar to DNA processing during conjugative DNA transfer between bacteria.